Testing an integrated approach for monitoring Colletotrichum nymphaeae in soil samples
DOI:
https://doi.org/10.7868/S3034519726010102Keywords:
Colletotrichum nymphaeae, soil samples, DNA extraction from soil, real time PCR, phytosanitaryAbstract
This study presents a comparative assessment of two main methodological approaches for the detection and quantification of the phytopathogenic fungus Colletotrichum nymphaeae in soil: the cultural method using nutrient media and real-time PCR. The main purpose of the work was to evaluate and compare the sensitivity and practical limitations of these methods in relation to different soil types. The cultural method was optimized by testing various degrees of dilution of soil suspensions and comparing the efficiency of three nutrient media. In parallel, DNA extraction from contaminated soil using a commercial kit was tested, followed by identification using real-time PCR with specific primers. The results demonstrate that the efficiency of the cultural method depends on both the soil type and the selected nutrient medium. The PCR method showed significantly higher sensitivity, allowing for the detection of the pathogen at concentrations below the detection threshold of the cultural method. However, it is sensitive to the inhibitory effects of organically rich soils. The obtained data emphasize the need for an integrated approach. This study provides practical recommendations for monitoring C. nymphaeae, which contributes to improving the accuracy of phytosanitary diagnostics and the development of plant protection strategies.
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