MOLECULAR-GENETIC CHARACTERIZATION OF HAPLOID SUGAR BEET LINES

Abstract

Aim of the investigations is to carry out molecular-genetic study of sugar beet haploid plants-regenerants obtained by direct regeneration under in vitro culture. Results of these sugar beet lines’ molecular evaluation according to five microsattelite loci (SSR-markers – Unigenes:  24552, 14805, 16898, 22373 and 27833) and three minisattelites (TRs). As a result of the studies, experimental data characterizing initial sugar beet lines according to these molecular-genetic markers and allowing their clear differentiation have been obtained. Genetical distances (Euclidean) between the studied genotypes have been determined. The obtained sugar beet microclones have been clustered, and unique DNA-profiles for these breeding individuals have been made. From the results of the conducted molecular-genetic analysis of haploid plants-regenerants, it has been determined that DNA-markers of sugar beet mitochondrial genome belonging to the family of TRs minisatellites (TR1 and TR3) allow high-reliable identification of microclonal plants as MS and O-type forms. It has been revealed that plants-pollinators (sterility maintainers) of O-type are characterized by presence of DNA-amplicons: 700 bp in length when using the primer TR1 and 500 bp in length when using the primer TR3. The fragments of 400 bp are typical of MS plants-regenerants.