IDENTITY OF RESISTANCE GENES TO RACE G OF BROOMRAPE IN SOME LINES OF SUNFLOWER

Abstract

One of the most dangerous biotic factors that reduce the sunflower productivity is its infection by an obligate parasite of higher plants - broomrape (Orobanche cumana Wallr.). Over the past few years, a number of inbred lines of various origins that are immune to race G have been developed at V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK). To combine different genes in the same sunflower genotype, it is necessary to determine their allelism.  The research material was the sunflower lines – donors of resistance to race G of broomrape: RG, RGP1, RGP2, RGB, RGL1, RGL2, RGM. The identification of the race of broomrape seeds by means of lines-differentiators showed that the seeds were collected from the plants of race G. Hybrids F₁ and F₂ were obtained by the VNIIMK method. The mathematical processing of the segregation results was conducted by means of ?² criterion of correspondence of the actual segregations to the theoretically expected ones in dihybrid crossings. The research results showed that, overall, the parent lines were affected by broomrape to a small degree. During the test for allelism, seven generations of F₁ and F₂ were obtained from pair crossing of resistant lines with each other. The tested hybrids F₁ were either not affected by the parasite, or were affected to a small degree, which allowed us to classify sunflower plants as resistant. To determine the identity of resistance genes in the lines of RG with RGP1, RG with RGM, RG with RGL1, RG with RGB, RGP2 with RGL2, RGM with RGL1, RGB with RGL1, we analyzed the F₂ progeny from these hybrids. The test for allelism of the resistance genes to race G of these lines showed their identity, with the possibility of some distortion of the phenotypic manifestation of the trait in various crossing combinations.