INNOVATIVE WAYS FOR PRE-CLINICAL DIAGNOSTICS OF PUERPERAL METRITIS IN COWS USING THE «ELECTRONIC NOSE» DEVICE

Abstract

The article discusses the possibilities of using the device with chemical sensors - "electronic nose" "MAG-8" for the early diagnosis of postpartum endomyometritis in dairy cows in connection with clinical data and indicators of immunobiochemical status. Diagnostic accuracy of the use of the “Electronic Nose” instrument has been determined in relation to cervical-vaginal samples obtained 1-3 and 6-8 days after calving from cows with the physiological course of the postpartum period and its complications. The established differences in the morphobiochemical and immunological status of cows during the early postpartum period, subsequently fallen ill with metritis, indicate a decrease in the immunological resistance of their organism against the background of increased endogenous intoxication and lipid peroxidation due to the microbial pressure. At the same time, the development of the inflammatory process begins in the uterine cavity (1-3 days after calving), and then its manifestation follows (days 6-8). This is proved by the indicators of the volatile fraction of cervical mucus in cows predisposed to metritis, in which a decrease in the content of aliphatic acids by 14.3-22.7%, short-chain amines - by 8.4-9.2%, with an increase in the level of alcohol, ketones of high molecular weight - by 5.2-6.0%, the occurrence of ammonia, sulfur-containing compounds were stated. The marked changes in the qualitative and quantitative composition of the gas-phase equilibria in cows with metritis on days 1-3 and 6-8 of the postpartum period were similar and could be used as markers for early diagnosis of the inflammatory process development in the uterus.