Methods of the intestinal digestion poultry studying
DOI:
https://doi.org/10.31857/vrsn/2017/5/25-27Keywords:
meat-type chicken, duodenal digesta, duodenal fistula, soybean product, digestive enzymesAbstract
A method of evaluation of enzymatic activities in the duodenal digesta in growing meat-type chicken fed diets including ingredients with different nutritive value and digestibility is presented. The study was performed on 10 Plymouth Rock pullets (10-13 weeks of age) with chronic duodenal fistulae. Diets with higher nutritive density were found to increase the duodenal activities of amylolytic and lipolytic enzymes more than 2-fold. However, when full-fat soybean was substituted for sunflower cake (resulting in increase in dietary crude protein by 1.93%) the duodenal protease activity remained unchanged. These data evidence that the secretion of the digestive enzymes to duodenal digesta can finely adjust to feed quality including the adjustment to protein and amino acid digestibility in any specific diet. Chronic physiological experiments can be a comprehensive method in the studies on poultry nutrition; these can help in the research of the effects of different diets and additives on the digestive processes and nutrient digestibility.
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9. R e n, L.Q. Effects of dietary protein source on the digestive enzyme activities and electrolyte composition in the small intestinal fluid of chickens /L.Q. R e n, F. Z h a o , H.Z. T a n at all. //Poultry Sci., 2012, 91(7): 1641-1646 (doi: 10.3382/ps.2011-02081).
10. Rothman S., Conservation of digestive enzymes /S. Rothman, C. Liebow, L.Isenman at all.//Phsyol. Rev., 2002. 82:1-18. DOI 10.1152/physrev. 00022.2001.
2. Вертипрахов, В.Г. Активность ферментов поджелудочной железы у цыплят-бройлеров на разных этапах пищеварения /В.Г.Вертипрахов, А.А.Грозина, А.М.Долгорукова //Сельскохозяйственная биология,2016.-Т.51.-№4.-С.509-515.
3. Мерина-Глузкина, В.М. Сравнительная оценка сахарифицирующего и декстректирующего методов при определении активности амилазы крови здоровых и больных острым панкреатитом/В.М.Мерина-Глузкина//Лабораторное дело.-1965.-№3.-С.-142-146.
4. Никонов, И.Н. Аминокислотный состав комбикормов и микрофлора кишечника кур-несушек/И.Н. Никонов//Зоотехния.-2012.-№11.-27-28.
5. Фисинин, В.И. Панкреатическая секреция и усвоение аминокислот в кишечнике кур при разных источниках белка в рационе/В.И.Фисинин, В.Г.Вертипрахов, А.А.Грозина, Л.В.Хасанова//Сельскохозяйственная биология.-2017.-Т.52.-№2.-С.374-381.
6. Японцев, А.Э. Сравнение подходов к определению усвояемости аминокислот /А.Э. Японцев//Птицеводство.-2016.-№2.-С.35-37.
7. Valchev, I. Impaired pancreatic in mulard ducks with experimental aflatoxicosis /N. Grozeva, D. Kanakov, Ts. Hriston at all. //Agrocultural Science and Technology Vol.5.-No 4.-2013. -Р.394-399.
8. Mikhailova, A.G. Cloning, sequencing, expression, and characterization of thermostability of oligopeptidase B from Serratia proteamaculans, a novel psychrophilic protease /A.G. Mikhailova, R.F. Khairullin, I.V. Demidyuk at all. //Protein Expression and Purification, 2014.-93.-Р. 63–76.
9. R e n, L.Q. Effects of dietary protein source on the digestive enzyme activities and electrolyte composition in the small intestinal fluid of chickens /L.Q. R e n, F. Z h a o , H.Z. T a n at all. //Poultry Sci., 2012, 91(7): 1641-1646 (doi: 10.3382/ps.2011-02081).
10. Rothman S., Conservation of digestive enzymes /S. Rothman, C. Liebow, L.Isenman at all.//Phsyol. Rev., 2002. 82:1-18. DOI 10.1152/physrev. 00022.2001.
LIST OF SOURCES
1. Batoev, C.Zh. Fotometricheskoe opredelenie aktivnosti proteoliticheskih fermentov v podzheludochnoj zheleze, soke po umen'sheniju koncentracii kazeina/ C.Zh. Batoev// Sb. nauch. tr. Burjat. SHI (Ulan-Udje),1971,№25.-S. 122–126.
2. Vertiprahov, V.G. Aktivnost' fermentov podzheludochnoj zhelezy u cypljat-brojlerov na raznyh jetapah pishhevarenija /V.G.Vertiprahov, A.A.Grozina, A.M.,Dolgorukova //Sel'skohozjajstvennaja biologija, 2016.-T. 51.-№4.-S.509-515.
3. Merina-Gluzkina, V.M. Sravnitel'naja ocenka saharificirujushhego i dekstrektirujushhego metodov pri opredelenii aktivnosti amilazy krovi zdorovyh i bol'nyh ostrym pankreatitom /V.M.Merina-Gluzkina//Laboratornoe delo.-1965.-№3.-S.-142-146.
4. Nikonov, I.N. Aminokislotnyj sostav kombikormov i mikroflora kishechnika kur-nesushek /I.N. Nikonov//Zootehnija.-2012.-№11.-27-28.
5. Fisinin, V.I. Pankreaticheskaja sekrecija i usvoenie aminokislot v kishechnike kur pri raznyh istochnikah belka v racione / V.I.Fisinin, V.G.Vertiprahov, A.A.Grozina, L.V.Hasanova // Sel'skohozjajstvennaja biologija.-2017.- T.52.-№2.-S. 374-381.
6. Japoncev, A.Je. Sravnenie podhodov k opredeleniju usvojaemosti aminokislot/A.Je. Japoncev//Pticevodstvo.-2016.-№2.-S.35-37.
7. Valchev, I. Impaired pancreatic in mulard ducks with experimental aflatoxicosis /N. Grozeva, D. Kanakov, Ts. Hriston at all. //Agrocultural Science and Technology Vol.5.-No 4.-2013. -Р.394-399.
8. Mikhailova, A.G. Cloning, sequencing, expression, and characterization of thermostability of oligopeptidase B from Serratia proteamaculans, a novel psychrophilic protease /A.G. Mikhailova, R.F. Khairullin, I.V. Demidyuk at all. //Protein Expression and Purification, 2014.-93.-Р. 63–76.
9. R e n, L.Q. Effects of dietary protein source on the digestive enzyme activities and electrolyte composition in the small intestinal fluid of chickens /L.Q. R e n, F. Z h a o , H.Z. T a n at all. //Poultry Sci., 2012, 91(7): 1641-1646 (doi: 10.3382/ps.2011-02081).
10. Rothman S., Conservation of digestive enzymes /S. Rothman, C. Liebow, L.Isenman at all.//Phsyol. Rev., 2002. 82:1-18. DOI 10.1152/physrev. 00022.2001.
Additional Files
Published
2025-12-26
Issue
Section
SCIENTIFIC STUDIES
How to Cite
Fisinin, V. I., Vertiprakhov, V. G., Grozina А. А., & Svitkin, V. S. (2025). Methods of the intestinal digestion poultry studying. Vestnik of the Russian Agricultural Science, 5, 25-27. https://doi.org/10.31857/vrsn/2017/5/25-27
