Mycelia fungi - promising source of hydrolasas and valuable polymers

Abstract

Investigated the biosynthetic capacity of filamentous fungus Aspergillus oryzae strains RCAM 01133 and RCAM 01134 with respect to hydrolytic enzymes. It is found that the level of - amylase activity of strain RCAM 01133 inthe culture liquid (CL) higher than the level of enzyme activity of strain RCAM 01134 on 300 %, chitinase - on 70 %, mannanase - on 37 %, while the proteolytic activity of the fungus A.oryzae RCAM 01133 was significantly lower (30 times). Studied the content of residual intracellular enzymes in biomass of micromycetes A oryzae RCAM 01133. The highest content of amylase in cell - bound form fell on the first day - 48 % of the total enzyme activity. On the second day there was a decrease level of residual activity of intracellular enzyme: the amylase - to 31 %, chitinase - 33 %, mannanase - 29 %. Investigated the biochemical composition of the biomass of the fungus Aspergillus oryzae RCAM 01133 - producing a-amylase, ?-glucanases, chitinases and mannanase for dietary supplements. It is found that the maximum level corresponds to the formation of protein - 24,5 %, polysaccharides - 32,5 %. Investigated the amino acid composition of proteins biomass of micromycetes confirmed high levels of essential amino acids (55 - 56 % of total amino acids). Studied the possibility of using the biomass of the fungus Aspergillus oryzae RCAM 01133 as a promising source of biologically valuable polymers. The data obtained allow to consider the biomass of the fungus as a substrate containing protein enriched in essential amino acids, polysaccharides and residual enzymes for protein - carbohydrate supplementation.