Identification of the rust fungus parasitizing on field pea in the Orel region
Keywords:
field peas, rust fungi, pathological process, DNA, stipules, primers, test systems, molecular diagnosticsAbstract
On the pea sowing in the Orel region for years is diagnosticate a disease caused by rusty fungi of the genus Uromyces. Guided by observations of the life cycle and the hosts circle of the rust fungus that colonized the pea sowings, and partly the morphological features of Telio and Urediniospors, we assumed that the responsible for epiphytoty is the one-host fungus U. fabae f. pisi-sativae (specialized form of fungus U. fabae). To confirm the hypothesis, molecular genetic methods were used- phylogenetic analysis of genomes and diagnostics of plant diseases using PCR. However, in NCBI, the nucleotide base which is traditionally used to determine the systematic position of an organism in a hierarchy similar to it and to create diagnostic tests, similar a taxonomic unit is absent. Operating the collected information in the GenBank, we identified three clusters of rust fungi which parasitizing on peas. This investigation illustrates the possible differences that arise when using different approaches to characterize one organism.
References
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4. Хохряков, М.К. Определитель болезней растений / М.К. Хохряков, Т.Л.Доброзракова, К.М. Степанов, М.Ф. Летова. - СПб: изд. Лань, 2003. // Khokhryakov, M.K. Opredelitel‘ bolezney rasteniy [Plant diseases guide] / M.K. Khokhryakov, T.L. Dobrozrakova, K.M. Stepanov, M.F. Letova. - SPb: izd. Lan‘, 2003.
5. Arthur JC (1905) Terminology of the spore-structures in the Uredinales. Botanical Gazette (Chicago, Ill.) 39, 219- 222. doi:10.1086/328608.
6. Barilli E., Satovic Z., Sillero J.C., Rubiales D., Torres A.M. (2011): Phylogenetic analysis of Uromyces species infecting grain and forage legumes by sequence analysis of nuclear ribosomal Internal Transcribed Spacer region. Journal of Phytopathology, 159: 137-145.
7. Craigie, J. H. 1927. Discovery of the function of the pycnia of the rust fungi. Nature 120: 765-767.
8. Dietel P (1900) Uredinales. In ‘Die nat?rlichen Pflanzenfamilien I’(Eds A Engler, K Prantl) pp. 24-81. (Verlag Wilhelm Engelmann: Leipzig, Denmark).
9. Doyle J.J. and Doyle J.L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. 1987, Phyto chemistry Bulletin 19:11-15.
10. Kurkela T, Hanso M, Hantula J (1999) Differentiating cha racteristics between Melampsoridium rusts infecting birch and alder leaves. Mycologia 91, 987-992.
11. Leppik EE (1972) Evolutionary specialization of rust fungi (Uredinales) on the Leguminosae. Annales Botanici Fennici 9, 135-148.
12. Maier W., Begerow D., WeiЯ M., and Oberwinkler F. Phylogeny of the rust fungi: an approach using nuclear large subunit ribosomal DNA sequences. 2003. Canadian Journal of Botany, 81, 12-23.
13. Moricca S, Kasuga T, Mitchelson K, Ragazzi A, Dia mandis S (1996) Heterogeneity in intergenic regions of the ribosomal repeat of the pine-blister rusts Cronartium flaccidum and Peridermium pini. Current Genetics 29, 388-394.
14. Roy, B. A. 1993. Floral mimicry by a plant pathogen. Natu re 362: 56-58.
15. Roy, B. A. 1994. The use and abuse of pollinators by fungi. Trends in Ecology and Evolution 9: 335-339.
16. Roy BA, Vogler D, Bruns T, Szaro TM (1998) Cryptic species in the Puccinia monoica complex. Mycologia 90, 847-854.
17. Savile D.B.O. Fungi as aids in higher plant classification. 1979, Botanical Review 45, 377-503.
18. Singh KB, Singh A, Raina SN, Singh AK, Ogihara Y. (2002) Ribosomal DNA repeat unit polymorphism and heritability in peanut (Arachis Hypogea L.) accessions and related wild species. Euphytica 123:211-220.
19. Singh A, Devarumath RM, RamaRao S, Singh VP, Raina SN. (2008) Assessment of genetic diversity, and phylogenetic relationship based on ribosomal DNA repeat unit length variation and Internal Transcribed Spacers (ITS) sequences in chickpea (Cicer arietinum) cultivars and its wild species. Genet Resour Crop Evol 55:65-79.
20. Tulasne LR, Tulasne C (1847) M?moire sur les Ustila gin?es compares aux Ur?din?es. Annales des Sciences naturelles, Botanique s?r 3, 12-127.
21. Tulasne LR (1854) Second m?moire sur les Ur?din?es et les Ustilaginйes. Annales des Sciences naturelles, Bota nique s?r 4, 77-196.
22. Vogler DR, Bruns TD (1998) Phylogenetic relationships among the pine stem rust fungi (Cronartium and Pe ridermi um spp.). Mycologia 90, 244-257.
23. Zambino PJ, Szabo LJ (1993) Phylogenetic relationships of selected cereal and grass rusts based on rDNA sequence analysis. Mycologia 85, 401–414.
Additional Files
Published
2017-12-14
Issue
Section
SCIENTIFIC STUDIES
How to Cite
Karakotov, S. D., Arshava, N. V., Bozhko, K. N., Shchedrin, V. A., & Staheev, A. A. (2017). Identification of the rust fungus parasitizing on field pea in the Orel region. Vestnik of the Russian Agricultural Science, 4, 39-45. http://www.vestnik-rsn.ru/index.php/vrsn/article/view/36
