Analysis of antioxidant activity in products of processing red varieties of grapes in vitro, in vivo
Keywords:
grape polyphenols, antioxidant activity, bioluminescent bacteria, biotesting, wine, grape concentratesAbstract
Experimental data on the antioxidant activity of grape juice, grape concentrates and wine from grapes of Cabernet Sauvignon, Merlot and Saperavifrom Crimea and Krasnodar regions was presented. It was found that antioxidant activity for all products in standard Trolox method can be estimated by the equation: Y=0,53627+0,1395X+0,080439X2- 0,(K)064708X3, with a correlation coefficient r=0,9952, where Y - antioxidant activity, g/dm3 by Trolox method; X -mass concentration of phenolic substances on the Folin-Ciocalteu g/dm3. The equation is valid for Y (0,76 ч 196,22); X (1,0 ч 82,67). The results of biological testing wines Cabernet Sauvignon, Merlot & Saperavi and polyphenol concentrates Enoant, Enoant Premium & Fenokor on the biological model of bioluminescent bacteria Photobacterium leiognathi Sh1 demonstrated the applicability of bioassay to assess the antioxidant activity, which correlates well with the polyphenols concentration values and antioxidant activity by Trolox method.