IMPROVEMENT OF THE PROCESS OF CRYOPRESERVATION OF CATTLE SPERM WITH MOLECULAR HYDROGEN

Authors

  • M. N. Ivashchenko National Research Nizhny Novgorod State University named after N.I. Lobachevsky, Nizhny Novgorod State Agrotechnological University named after L.I. Florentyev
  • A. V. Deryuginа National Research Nizhny Novgorod State University named after N.I. Lobachevsky
  • A. A. Belov National Research Nizhny Novgorod State University named after N.I. Lobachevsky, Nizhny Novgorod State Agrotechnological University named after L.I. Florentyev
  • M. I. Latushko Production Association "Ural Optical and Mechanical Plant" named after E.S. Yalamov
  • P. S. Ignatiev Production Association "Ural Optical and Mechanical Plant" named after E.S. Yalamov

DOI:

https://doi.org/10.31857/S2500208225010146

Keywords:

cattle, molecular hydrogen, spermatozoa, cryopreservation, ATP, mobility, oxidative stress

Abstract

The effect of molecular hydrogen on the functional parameters of bovine sperm cells has been studied. The influence of molecular hydrogen on motility, ATP content, oxidative processes, viability and morphology of the acrosome in bull spermatozoa has been studied. The study was performed on sperm production of black-and-white cattle. The sperm was diluted with a sterile BioXcell medium (France). To analyze the effect of molecular hydrogen on bull spermatozoa, molecular hydrogen was added to the BioXcell medium. The analyzed parameters were studied in native sperm diluted with BioXcell medium, in sperm after deep freezing, as well as sperm subjected to deep freezing and pretreatment with molecular hydrogen. The addition of molecular hydrogen to the medium for diluting sperm contributed to an increase in cell mobility, increased energy metabolism and reduced oxidative stress of spermatozoa. The results obtained showed the need for an in-depth study of the effect of molecular hydrogen on the qualitative characteristics of sperm in cattle and, on this basis, to clarify the existing technological regulations for seed conservation.

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Published

2025-03-03

Issue

No. 1 (2025): January-February

Section

ЗООТЕХНИЯ

How to Cite

Ivashchenko, M. N., Deryuginа A. V., Belov, A. A., Latushko, M. I., & Ignatiev, P. S. (2025). IMPROVEMENT OF THE PROCESS OF CRYOPRESERVATION OF CATTLE SPERM WITH MOLECULAR HYDROGEN. Vestnik of the Russian Agricultural Science, 1, 63-66. https://doi.org/10.31857/S2500208225010146