BIOTECHNOLOGICAL METHODS FOR OBTAINING SWEET POTATO (IPOMOEA BATATAS L.) FORMS RESISTANT TO HYPOTHERMIC STRESS RESISTANCE

Abstract

The creation of new forms, hybrids and varieties of sweet potatoes (Ipomoea batatas (L.)) resistant to hypothermic stress is an urgent area of research. Studies on the cell selection of sweet potatoes for resistance to this stress have not been conducted in Russia and the world before. The aim of the work is to develop in vitro technology for obtaining resistant forms of sweet potato plants to positive low temperatures. The object of the study was sweet potato tubers of the Jewel variety. The primary explant was young leaves isolated from aseptic plants, which were cultivated on a nutrient medium containing mineral salts according to the MS recipe, as well as NAA at a concentration of 1 mg/l in combination with BAP 0.5 mg/l. Under these conditions, callus tissue was formed, which was later used in cell selection. The callus was grown in a thermostat at 14⁰C for 30 days. It was found that the presence of Mival or Crezacin preparations at a concentration of 150 mg/l in the nutrient medium has a significant positive effect on the viability of the callus culture under hypothermic stress (14⁰C). In these variants, cell viability was 56.1...68.5%, and in the control variant - 3.5...4.2%. Yam regenerant plants were obtained from stable cell cultures.